RT Master Mix for cDNA Generation
Reagent	Volume per reaction (μl)
10× RT Buffer	2.0
25× dNTP Mix	0.8
10× RT Random Primers	2.0
RT	1.0
Total Volume	5.8

cDNA generation PCR steps
Step	Cycles	Temp	Time
1	1	25°C	10 min
2	1	37°C	2 h
3	1	85°C	5 min
4		4°C	Infinity