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. 2020 Sep 15;1(2):100102. doi: 10.1016/j.xpro.2020.100102
REAGENT or RESOURCE SOURCE IDENTIFIER
Biological Samples Healthcare providers N/A

Critical Commercial Assays

Universal Transport Media (UTM) 3-mL collection kit with flexible minitip flocked swab BD 220531
MicroAmp™ Optical 384-Well Reaction Plate with Barcode Fisher Scientific 43-098-49
∗High Capacity cDNA Reverse Transcription Kit 1000 rxn Fisher Scientific 43-688-13
Synthetic SARS-CoV-2 RNA ATCC SD-3276
RNeasy Mini Kit (250) Qiagen 74106
∗TaqMan™ Fast Advanced Master Mix ThermoFisher 4444557
2019-nCoV CDC Emergency Use Authorization Kits IDT 110006606
MicroAmp Optical Adhesive Film ThermoFisher 4311971
†Taqpath qPCR Master Mix, CG ThermoFisher A47814
ˆTaqPath 1-Step Multiplex Master Mix (No ROX) ThermoFisher A28522
∗0.2 mL PCR 8-strip with indiv. attached dome caps USA Scientific 1402-2900
Proteinase K Roche 3115836001
Seal-Rite 1.5 mL graduated microcentrifuge tube, (500 tubes) USA Scientific 1615-5510
RNase away ThermoFisher 21-402-178
Nuclease-Free H2O Fisher Scientific BP2484100
Molecular Biology Grade Ethanol Millipore Sigma E7023

Software and Algorithms

GraphPad Prism version 7 GraphPad Software, La Jolla, CA www.graphpad.com
RStudio RStudio, Inc., Boston, MA www.rstudio.com
Script for automated analysis, reporting, and uploading of qRT-PCR data This paper https://github.com/TaylorMatte/Quant6-Covid_Analysis

Other

FDA approved BMC-CReM EUA submission This paper See File S1
QuantStudio 6 Flex Real-Time PCR System, 384-well, desktop ThermoFisher 4485701
∗Mastercycler Nexus X2 (software version 3.5.1) Eppendorf 6336000023