Biological Samples |
Healthcare providers |
N/A |
|
Critical Commercial Assays |
|
Universal Transport Media (UTM) 3-mL collection kit with flexible minitip flocked swab |
BD |
220531 |
MicroAmp™ Optical 384-Well Reaction Plate with Barcode |
Fisher Scientific |
43-098-49 |
∗High Capacity cDNA Reverse Transcription Kit 1000 rxn |
Fisher Scientific |
43-688-13 |
Synthetic SARS-CoV-2 RNA |
ATCC |
SD-3276 |
RNeasy Mini Kit (250) |
Qiagen |
74106 |
∗TaqMan™ Fast Advanced Master Mix |
ThermoFisher |
4444557 |
2019-nCoV CDC Emergency Use Authorization Kits |
IDT |
110006606 |
MicroAmp Optical Adhesive Film |
ThermoFisher |
4311971 |
†Taqpath qPCR Master Mix, CG |
ThermoFisher |
A47814 |
ˆTaqPath 1-Step Multiplex Master Mix (No ROX) |
ThermoFisher |
A28522 |
∗0.2 mL PCR 8-strip with indiv. attached dome caps |
USA Scientific |
1402-2900 |
Proteinase K |
Roche |
3115836001 |
Seal-Rite 1.5 mL graduated microcentrifuge tube, (500 tubes) |
USA Scientific |
1615-5510 |
RNase away |
ThermoFisher |
21-402-178 |
Nuclease-Free H2O |
Fisher Scientific |
BP2484100 |
Molecular Biology Grade Ethanol |
Millipore Sigma |
E7023 |
|
Software and Algorithms |
|
GraphPad Prism version 7 |
GraphPad Software, La Jolla, CA |
www.graphpad.com |
RStudio |
RStudio, Inc., Boston, MA |
www.rstudio.com |
Script for automated analysis, reporting, and uploading of qRT-PCR data |
This paper |
https://github.com/TaylorMatte/Quant6-Covid_Analysis |
|
Other |
|
FDA approved BMC-CReM EUA submission |
This paper |
See File S1
|
QuantStudio 6 Flex Real-Time PCR System, 384-well, desktop |
ThermoFisher |
4485701 |
∗Mastercycler Nexus X2 (software version 3.5.1) |
Eppendorf |
6336000023 |