| cDNA generation PCR steps | |||
|---|---|---|---|
| Step | Cycles | Temp | Time |
| 1 | 1 | 25°C | 10 min |
| 2 | 1 | 37°C | 2 h |
| 3 | 1 | 85°C | 5 min |
| 4 | 4°C | Infinity | |
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