| Two-Step Singleplex qPCR Program | |||
|---|---|---|---|
| Step | Cycles | Temp | Time |
| Preamplification (UNG inactivation) | 1 | 25°C | 120 s |
| Preamplification (polymerase activation) | 1 | 95°C | 120 s |
| Amplification | 45 | 95°C | 3 s |
| 55°C | 30 s | ||
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