| One-Step Singleplex qPCR Master Mix Recipe | |
|---|---|
| Reagent | Volume per reaction (μl) |
| 4× TaqPath 1-Step RT-qPCR Master Mix | 5 |
| Combined primer/probe mix | 1 |
| Nuclease-free H2O | 11.50 |
| Final total RXN volume | 20 |
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