| One-step singleplex qPCR program | ||
|---|---|---|
| Cycles | Temp | Time |
| 1 | 25°C | 2 min |
| 1 | 50°C | 15 min |
| 1 | 95°C | 2 min |
| 40 (including) | 95°C | 3 s |
| 60°C | 30 s | |
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