Figure 2.
Day of Induction
Bioreactors were inoculated with 8 × 107 cells and induced 4–7 days later in DMEM with 10% FBS and 2 mM GlutaMAX. The pH set point was adjusted to 6.8 after induction in all reactors. Culture continued for 11 days post-induction in the reactor induced 4 days after seeding, for 10 days in the reactor induced 5 days after seeding, for 9 days in the reactor induced 6 days after seeding, and for 8 days in the reactor induced 7 days after seeding. (A) Cell density on each day post-induction in reactors induced 4–7 days after inoculation. (B) Glucose consumption per day post-induction for reactors induced 4–7 days after inoculation. (C) Viral yield on each day post-induction for reactors induced 4–7 days after inoculation.