. 2020 Sep 14;10:15057. doi: 10.1038/s41598-020-71895-x

Table 2.

qPCR primer set (murine) of all target genes tested.

Symbol	Gene name	Protein function	Primer sequence (F: 5′-3′/R: 3′-5′)
ApoE	Apolipoprotein E	Major apoprotein of the chylomicron, essential for the normal catabolism of lipoprotein constituents with triglycerides	F: TGGTGTTCAAAGACAATTTTTCCCT R: CCACTCGAGCTGATCTGTCAC
Atf4	Activating transcription factor 4	Transcription factor, also characterised as the cAMP-response element binding protein 2 (Creb2)	F: CACTGGCGAGTGTAAGGAGC R: TTCTTCCCCCTTGCCTTACG
Creb1	cAMP responsive element binding protein 1	Phosphorylation-dependent transcription factor	F: CCCTGCCATCACCACTGTAA R: GGTTAATGTCTGCAGGCCCT

Symbol

Gene name

Protein function

Primer sequence (F: 5′-3′/R: 3′-5′)

ApoE

Apolipoprotein E

Major apoprotein of the chylomicron, essential for the normal catabolism of lipoprotein constituents with triglycerides

F: TGGTGTTCAAAGACAATTTTTCCCT

R: CCACTCGAGCTGATCTGTCAC

Atf4

Activating transcription factor 4

Transcription factor, also characterised as the cAMP-response element binding protein 2 (Creb2)

F: CACTGGCGAGTGTAAGGAGC

R: TTCTTCCCCCTTGCCTTACG

Creb1

cAMP responsive element binding protein 1

Phosphorylation-dependent transcription factor

F: CCCTGCCATCACCACTGTAA

R: GGTTAATGTCTGCAGGCCCT