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Summary of key findings and recommendations for reference genes in analysed tissues.
| Days post trauma | Reference genes recommended | |
|---|---|---|
| Primary organs | ||
| Bone/callus | d3 | Hprt + Gapdh + B2m |
| d7 | Hprt + Ppia | |
| Hypothalamus | d3 | Hprt + Actb |
| d7 | Hprt + Gapdh | |
| Secondary organs | ||
| White adipose tissue (WAT) | d3 | Ppia + Psmb2 |
| d7 | Ppia + Psmb2 | |
| Liver | d3 | Ppia + B2m/Hprt |
| d7 | Ppia + B2m/Hmbs | |
| Muscle | d3 | Ppia + Hprt |
| Spleen | d3 | Ppia + Hprt |
