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. 2020 Sep 14;10:15046. doi: 10.1038/s41598-020-71773-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rolling circle amplification of circular DNA by B35-HhH. (A) Schematic representation of the rolling circle amplification (RCA) process of a singly primed M13 DNA, which can give rise to a large single-stranded DNA product due to the intrinsic strand displacement capacity. (B) Alkaline gel electrophoresis of DNA products synthesised by B35DNAP and B35-HhH in RCA assays. λ-HindIII DNA ladder was used as a size marker (lane M). Full-length M13 DNA size is indicated with a black arrow.