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. 2020 Sep 14;10:15046. doi: 10.1038/s41598-020-71773-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spectra of single base changes by B35DNAP and B35-HhH in DNA amplification experiments. Bases are colored in red (A), blue (C), green (T), and orange (G). Base insertions, deletions, and substitutions by B35DNAP or B35-HhH are indicated above and below the sequence, respectively, according to the legend, as inverted triangles (▼), greek letter delta (Δ), and the mutated base, respectively, maintaining the same color code. Position 1 is the first transcribed nucleotide of the lacZ gene.