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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: Nat Protoc. 2020 Apr 1;15(5):1649–1672. doi: 10.1038/s41596-020-0300-1

Table 3 |.

Monomer solution preparation from the stock solutions

Chemical Stock concentration* Amount (ml) Final concentration*
Sodium acrylate 38 2.25 8.6
Acrylamide 50 0.5 2.5
N,N’-Methylenebisacrylamide 2 0.50 0.10
Sodium chloride 29.2 4 11.7
PBS 10x 1 1x
Water 1.15
Total 9.4**
*

All concentrations are in g/100 ml, except PBS

**

9.4/10 ml (1.06x), with the remaining 6% volume brought up by initiator, accelerator and inhibitor.