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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: Nat Protoc. 2020 Apr 1;15(5):1649–1672. doi: 10.1038/s41596-020-0300-1

Table 4 |.

Example combination of filter sets for four color imaging

Fluorophore Excitation (nm) Dichromatic (nm) Emission (nm)
DAPI 325–375 400 435–485
Alexa Fluor 488 451–490 497 502–542
Alexa Fluor 546 532–588 595 604–679
Atto 647N 590–650 660 663–738