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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Toxicol Lett. 2019 Sep 25;317:1–12. doi: 10.1016/j.toxlet.2019.09.013

Fig. 7.

Fig. 7.

3-D printer-emitted particles collected in the cell culture medium increase apoptosis and necrosis. SAEC were stained with a cocktail consisting of 2 μL of Apopxin™ Red apoptosis stain, 1 μL of 200X Nuclear Green™ DCS1 necrotic stain, and 1 μL of CytoCalcein™ Violet 450 cytoplasmic stain. (A) Representative confocal images of untreated cells, and cells exposed to background, PC, and ABS collected emissions for 24 h. Quantitative apoptosis and necrosis analysis of PC (B) and ABS (C) using high content screening image analysis. Magnification at 10× and scale bars are at 50 μm for all images. Experiments were performed in three independent experiments with n = 6 replicates each. The regressions lines illustrate a significantly (p < 0.0001) increased dose-response relationship between apoptotic or necrotic events and the numbers of the PC and ABS 3-D printer-emitted particles. The shaded area represents the 95% confidence interval around the regression line.