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. 2020 Sep 14;18:124. doi: 10.1186/s12915-020-00854-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Over-expression of wild-type Pten, but not lipid phosphatase dead Pten, extends lifespan and delays development. a, b Survival curves show a extended lifespan for w^Dah;Actin-GeneSwitch/UAS-Pten;+ flies and b unchanged lifespan for w^Dah;Actin-GeneSwitch/UAS-Pten-G137E;+ flies reared on food containing 200 μM RU-486 (induced, red and pink curves) from 2 days of age compared to sibling flies reared on vehicle control food (uninduced, black and grey curves). c, d Survival curves show c extended lifespan for w^Dah;UAS-Pten/+;repo-GAL4/+ flies (red) compared to control w^Dah;+;repo-GAL4/+ (blue) and w^Dah;UAS-Pten/+;+ (black) flies, and d unchanged lifespan for w^Dah;UAS-Pten-G137E/+;repo-GAL4+ flies (pink) compared to control w^Dah;+;repo-GAL4/+ (blue) and w^Dah;UAS-Pten-G137E/+;+ (grey) flies. For all lifespans, n > 130 deaths counted per group. e, f Egg-to-adult timing shows e delayed development for w^Dah;UAS-Pten/+;repo-GAL4/+ flies (red) compared to control w^Dah;+;repo-GAL4/+ (blue) and w^Dah;UAS-Pten/+;+ (black) flies, and f no delay in development for w^Dah;UAS-Pten-G137E/+;repo-GAL4/+ flies (pink) compared to control w^Dah;+;repo-GAL4/+ (blue) and w^Dah;UAS-Pten-G137E/+;+ (grey) flies. n > 55 flies per group. Note that the repo-GAL4/+ curves in c, d and e, f are identical, as these experiments were run in parallel. g, h qPCR from head RNA shows g over-expression of Pten in both w^Dah;UAS-Pten/+;repo-GAL4/+ and w^Dah;UAS-Pten-G137E/+;repo-GAL4/+ flies using primers outside of the G137 region and h over-expression of wild-type Pten in only w^Dah;UAS-Pten/+;repo-GAL4/+ flies using primers designed to amplify the wild-type sequence for G137. n = 4 replicates of 10–12 heads per replicate. p values are from log-rank tests (a–f) or Bonferroni’s multiple comparison (g, h) tests versus control group of that colour