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. 2020 Sep 14;39:186. doi: 10.1186/s13046-020-01697-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Downregulation of hsa_circ_0001944 suppressed BC proliferation in vitro. a RT-qPCR detection showing the expression of hsa_circ_0001944 in different BC cell lines. Data are presented as mean ± SD; ^***P < 0.001 vs. the SV-HUC-1 group. b RT-qPCR detection showing the expression of hsa_circ_0001944 in both T24 and UMUC3 cells after transfection with siRNA against hsa_circ_0001944 or negative control. Data are presented as mean ± SD; ^***P < 0.001 vs. the NC. c Flow cytometry detection showing the percentages of cells in G1, S, or G2 phase in both T24 (C) and UMUC3 (D) cells. d and e CCK8 assays were used to evaluate cell proliferation. Data are presented as mean ± SD; ^***P < 0.001 vs. NC. f and g Colony formation assay showing proliferation in both T24 and UMUC3 cells. Data are presented as mean ± SD; ^***P < 0.001 vs. NC. h and i Edu assays were used to evaluate cell proliferation. Data are presented as mean ± SD; ^***P < 0.001 vs. NC