Fig. 3.

Inner medullary P2Y₂ and P2Y₄ receptor mRNA expression and protein abundance. Relative mRNA expression and total protein abundance of P2Y₂ (a, c) and P2Y₄ (b, d) receptor in the inner medulla from male, ovary-intact female, and OVX Sprague Dawley rats (representative Western blots are presented). Gene expression and protein abundance values represent fold change from ovary-intact female levels. n = 4-8 rats in each group. Statistical comparisons performed using one-way ANOVA followed by Sidak’s post hoc tests. ANOVA results: P2Y₂ mRNA: P = 0.004, F = 7.1; P2Y₄ mRNA: P = 0.007, F = 8.0; P2Y₂ protein: P = 0.02, F = 4.9; P2Y₄ protein: P = 0.5, F = 0.7