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. 2020 Sep 14;11:52. doi: 10.1186/s13293-020-00329-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — E₂ promotes P2Y₂ and P2Y₄ receptor mRNA expression in the inner medullary collecting duct cells (IMCD-3). P2Y₂ (a) and P2Y₄ (b) receptor mRNA expression in IMCD-3 cells incubated with E₂ (1, 10, 100, 1000 nM) or vehicle for 24 h. n = 3-6 in each group. Statistical comparisons performed using one-way ANOVA followed by Dunnett’s post hoc tests. ANOVA results: P2Y₂ mRNA: P = 0.04, F = 3.7; P2Y₄ mRNA: P = 0.01, F = 6.1