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. 2020 Sep 8;20(5):210. doi: 10.3892/ol.2020.12073

Figure 3.

Figure 3.

miR-150 downregulates CDKN1B mRNA and p27Kip1 expression. Total RNA and protein were extracted from transfected HeLa cells. (A) HeLa cells were transfected with miRNA controls or miR-150 mimics. (B) HeLa cells were transfected with miRNA controls or miR-150 inhibitors. Average cycle threshold value for the endogenous control RNU6B was used to normalize the raw cycle threshold data and calculate ΔCq. The 2−ΔΔCq method was used to determine the relative expression levels of miR-150. Significant differences were assessed using the Mann-Whitney U test. (C) The average cycle threshold value for the housekeeping gene GAPDH was used to normalize the raw cycle threshold data and calculate ΔCq. The 2−ΔΔCq method was used to determine the relative expression levels of CDKN1B. Significant differences were assessed using Dunn's test. **P<0.01. Bars represent the mean ± standard deviation of three experiments. (D) Representative western blot analysis shows p27Kip1 protein expression in transfected HeLa cells. β-actin was used as a loading control for western blot analysis. miR, microRNA.