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. 2020 Sep 9;20(5):215. doi: 10.3892/ol.2020.12078

Figure 3.

Figure 3.

STAT3 is a direct target gene of miR-4485. (A) Phosphorylated and total protein levels of STAT3, PI3K, AKT and mTOR and the ratio of p-STAT3/STAT3, p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR in A549 cells infected with or without H1N1. (B) Predicted binding sequences of STAT3 and miR-4485. A luciferase reporter assay demonstrated that STAT3 3′ untranslated region-WT was targeted by miR-4485 in 293T cells. (C) Reverse transcription-quantitative PCR confirmation of miR-4485 knockdown in A549 cells following transfection with miR-4485 inhibitor. (D) Phosphorylated and total protein levels of STAT3, PI3K, AKT and mTOR and the ratio of p-STAT3/STAT3, p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR in A549 cells transfected with miR-4485 mimic/inhibitor or mimic/inhibitor NC. The experiments were performed three times and the data are presented as the mean ± SD. **P<0.01 vs. control or mimic NC group; ##P<0.01 vs. inhibitor NC group. miR-4485, microRNA-4485; Mut, mutant; NC, negative control; p-, phosphorylated; WT, wild type.