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. 2020 Aug 27;23(9):101506. doi: 10.1016/j.isci.2020.101506

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Light-Dose-Dependent Control of PA-Gal4cc Transcriptional Activity

(A) Schematic representation of experimental conditions for testing the effects of the duration of blue light on-phase in the on-off cycle.

(B and C) Blue light duration-dependent increase in measured luciferase activities (B) and fold-increase of luciferase activity (Light/Dark) (C) in PA-Gal4cc transiently transfected HEK293T cells. The data represent mean values ± SD (n = 8) from three independent experiments.

(D) Schematic representation of experimental conditions for analyzing the effects of the number of applied blue light pulses.

(E and F) Light pulse number-dependent increase in measured luciferase activities (E) and fold-increase of luciferase activity (Light/Dark) (F) in PA-Gal4cc transiently transfected HEK293T cells. The data represent mean values ± SD (n = 7) from three independent experiments. The data represent mean ± SD. ∗p < 0.05; one-way ANOVA followed by Tukey's posthoc test.