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. 2020 Aug 27;23(9):101506. doi: 10.1016/j.isci.2020.101506

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Temporal Features of the PA-Gal4cc Transcriptional Activators

(A) HEK293T cell transfected with the PA-Gal4cc constructs and 5x UAS-Ub-NLS-luc2-Ascl1 3′ UTR reporter were exposed to a single blue light pulse. The timing of blue light exposure is indicated by vertical blue lines. The blue light was applied to cells 30 h after the transfection. The transcription on- and off-phases are highlighted in green and yellow, respectively.

(B and C) Using the single light pulse data set, kymograph analysis was used to determine the half-lives of the switch-on (B) and switch-off (C) kinetics of the PA-Gal4cc transcriptional activators. The data represent mean ± SD. ∗p < 0.05; one-way ANOVA followed by Dunnett's posthoc test (GAVPO versus each PA-Gal4cc). The rank order of the half-life of the switch-on/off kinetics between the PA-Gal4cc constructs was summarized in Figure S18.