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. 2020 Aug 27;23(9):101506. doi: 10.1016/j.isci.2020.101506

Figure 5.

Figure 5

Periodic Activation of the PA-Gal4cc Transcriptional Activators

(A–C) Transiently transfected HEK293T cells, in which PA-Gal4cc and 5x UAS-Ub-NLS-luc2-Ascl1 3′ UTR reporter had been introduced via lipofection, were repeatedly exposed to blue light pulses at 12- (A), 6- (B), or 3-h (C) intervals. The timing of blue light exposure is indicated by vertical blue lines. The first blue light illumination was initiated 24 h after the transfection. Experiments were repeated at least three times with similar results.