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. 2020 Aug 27;23(9):101506. doi: 10.1016/j.isci.2020.101506

Figure 6.

Figure 6

Light-Induced Gene Expression Control with PA-Gal4cc-Expressing Lentiviral Vectors

(A–F) The PA-Gal4ccE or GAVPO lentiviral vector-transduced HEK293T cells were repeatedly exposed to blue light pulses at 3- (A and D), 6- (B and E), or 12-h (C and F) intervals. The reporter construct consisted of 5x UAS, Ub-NLS-luc2, and Hes1 3′ UTR sequences. The timing of blue light exposure is indicated by vertical blue lines. Experiments were repeated at least three times with similar results.

(G and H) PA-Gal4ccE (G)- and GAVPO (H)-introduced HEK293T cells were exposed to a single blue light pulse.

(I and J) Using the single light pulse data set, kymograph analysis was used to determine the half-lives of the switch-on (I) and switch-off (J) kinetics of light-induced gene expression. The data represent mean ± SD. ∗p < 0.05; two-tailed Student's t test.