Figure 7.

Spatially Controlled Regulation of PA-Gal4cc Transcriptional Activators by Patterned Light Illumination

(A–D) Targeted cell populations were illuminated by patterned light generated by a digital mirror device (DMD). The patterned light, indicated by blue circles, was applied to HEK293T cells in which the PA-Gal4ccE (A and B) and PA-Gal4ccH (C and D) were transiently introduced by lipofection. (B and D) Light-induced reporter expression was quantified after patterned light illumination in the white circled regions. The timing of blue light exposure is indicated by blue arrowheads. Experiments were repeated at least three times with similar results. 86.0% ± 19.0% and 83.3% ± 28.9% showed light-induced reporter expressions in the PA-Gal4ccE- and PA-Gal4ccH-transfected cells, respectively. Scale bars, 100 μm.