Figure 3.

Leaf extract of Abeliophyllum distichum inhibits RANKL-induced NFATc1 expression. (A) Heatmap showing the reads per kilobase per million (RPKM) of c-Fos, p65 and Nfatc1. Numbers within the figure indicate RPKM values. (B) BMM cells were cultured for 3 days in the presence of M-CSF (30 ng/mL) and RANKL (100 ng/mL) in the absence or the presence of LE. Total RNA was isolated from cell lysates, and qRT-PCR was performed using primers specific for c-Fos, p65 and Nfatc1. The mRNA levels were normalized against an internal β-actin control. The results shown are mean values from three independent experiments; * p < 0.05. (C) Whole cell lysates were prepared from M-CSF/RANKL-treated BMMs with or without LE (10 μg/mL) for 0, 1, 2, and 3 days, and analyzed by immunoblotting with antibodies against NFATc1 or β-Actin.