Figure 4.

Leaf extract of Abeliophyllum distichum suppresses NFATc1 expression by blocking NFATc1, p65 and c-FOS-mediated transactivity. (A) BMMs were pre-treated with DMSO and LE (10 μg/mL) for 2 h, and stimulated with RANKL for 0, 5, 15, and 30 min. The cells were lysed and immunoblotted with antibodies against p-AKT, AKT, p-ERK, ERK, p-IκB, IκB, p-p38, p38, p-JNK, and JNK. (B) 293T cells were transiently transfected with the reporter plasmid Nfatc1-Luc along with c-Fos, p65, or Nfatc1 in the presence or absence of LE. Luciferase activity was measured after 24h post-transfection. Each bar represents the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.