Fig. 3.

UDCA Alleviates Markers of Cell Death in CHMP2B^Intron5 Models.

A. Effect of CHMP2B^Wildtype or CHMP2B^Intron5 ± UDCA (10 μM, 48 h) on the survival of mature cortical neurons. Data represents mean ± SEM analysed using two-way ANOVA followed by a Dunnett's (^⁎p < .050) and Tukey's multiple comparisons post hoc test (^#p < .05). 125 neurons analysed over three independent experiments.

B—C. Relative p53 abundance in the Drosophila CNS of third instar larvae (Wildtype vs CHMP2B^Intron5) raised on food supplemented with either vehicle or UDCA (600 μM). B. Quantification of 3 independent experiments blotting p53 from Drosophila lysates. Normalised against actin loading control and relative to vehicle treated wild types. ANOVA with post-hoc Dunnett's comparison to wild type controls ^⁎⁎⁎p < .001. C. Representative immunoblot of p53.

D, E. Relative Cleaved Death Caspase 1 (Dcp-1) abundance in the Drosophila CNS of third instar larvae (Wild type vs CHMP2B^Intron5) raised on food supplemented with either vehicle or UDCA (600 μM). D. Quantification of 3 independent experiments blotting cleaved Dcp-1 from Drosophila lysates. Normalised against actin loading control and relative to vehicle treated wild types. ANOVA with post-hoc Dunnett's comparison to wild type controls ^⁎p < .05. E. Representative immunoblot of cleaved Dcp-1.