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. 2020 Oct;144:105047. doi: 10.1016/j.nbd.2020.105047

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — UDCA Does Not Rescue Endosomal Perturbations in neurons expressing CHMP2B^Intron5.

A. Representative micrographs of RAB4 positive endosomes (early/recycling) in the CNS of Wild type and CHMP2B^Intron5 expressing (nSyb-Gal4) larvae raised on vehicle (ethanol) or UDCA (600 μM) supplemented food. Scale bars: main panel = 10 μm, inset = 2 μm.

B. Representative micrographs of mature neurons expressing FLAG-tagged CHMP2B^Wildtype or CHMP2B^Intron5 (FLAG, magenta) ± UDCA (10 μM, 48 h) stained for RAB4a (early/recycling endosome, green) protein. Nuclei counterstained with DAPI (blue). Scale bar = 5 μm.

C. Representative micrographs of RAB5 positive endosomes (early) in the CNS of Wild type and CHMP2B^Intron5 expressing (nSyb-Gal4) larvae raised on vehicle (ethanol) or UDCA (600 μM) supplemented food. Scale bars: main panel = 10 μm, inset = 2 μm.

D. Representative micrographs of mature neurons expressing FLAG-tagged CHMP2B^Wildtype or CHMP2B^Intron5 (FLAG, magenta) ± UDCA (10 μM, 48 h) stained for RAB5 (early endosome, green) protein. Nuclei counterstained with DAPI (blue). Scale bar = 5 μm.

E. Representative micrographs of the late endosome/lysosomal marker Spinster in the CNS of Wild type and CHMP2B^Intron5 expressing (nSyb-Gal4) larvae raised on vehicle (ethanol) or UDCA (600 μM) supplemented food. Scale bars: main panel = 10 μm, inset = 2 μm.

F. Representative micrographs of mature neurons expressing FLAG-tagged CHMP2B^Wildtype or CHMP2B^Intron5 (FLAG, magenta) ± UDCA (10 μM, 48 h) stained for RAB7 (late endosome, green) proteins. Nuclei counterstained with DAPI (blue). Scale bar = 5 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)