Figure 3.

HSP70 expression in cell lines and residual tumor tissues. HSP70 expression detected by (A) qRT-PCR and (B) western blot in four PDAC cell lines and normal HPDE cells. (C) Western blot and (D) qRT-PCR analysis for HSP70 expression after in vitro stimulation of MIA-PaCa-2 cells. (E) Representative immunohistochemistry staining of HSP70 in residual tumor tissues from orthotopic PDAC murine models days 1, 3 and 7 post-RFA treatment.

HSP70, heat shock protein 70; PDAC, pancreatic ductal adenocarcinoma; RFA, radiofrequency ablation; qRT-PCR, quantitative real-time polymerase chain reaction.

**p-value < 0.01, ***p-value < 0.001.