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. 2020 Sep 4;26:e924453-1–e924453-10. doi: 10.12659/MSM.924453

Figure 2.

Figure 2

Icariin reversed the effects of UA on Min6 cell viability and apoptosis. Min6 cells were treated with various concentration of icariin (0, 5, 10, 20 μM) for 2 h, followed by treatment with 5 mg/dl UA for another 24 h. (A) Cell viability and (B) apoptosis were assessed using MTT and flow cytometry analysis, respectively. (C) Quantity of apoptotic cells. (D) Protein levels of cleaved caspase-3 and pro-caspase-3 were examined using western blotting. ** P<0.01 compared with the control group; # P<0.05, ## P <0.01 compared with the UA group.