Table.
Table. Binding parameters of BMP-2 binding to immobilized BMPR-1A pretreated with naringin. The data obtained from measurements using immobilized BMPR-1A were fit to a kinetic model (1:1 Langmuir binding) from which the KD (bold) was calculated as kd/ka. All data represent the mean values of at least three repeated measurements using 6 different ligand concentrations.
| BMP-2 binding parameters | ||||||
|---|---|---|---|---|---|---|
| Pretreat with | ka (1/Ms) | kd (1/s) | Rmax (RU) | KA (1/M) | KD (M) | Chi2 |
| Without | 2.03e5 | 3.17e-4 | 363 | 6.41e8 | 1.56e-9 | 11.3 |
| Naringin | 1.77e5 | 3.82e-4 | 337 | 4.63e8 | 2.16e-9 | 10.7 |
| GSB | 4.22e5 | 5.67e-4 | 331 | 7.44e8 | 1.34e-9 | 106 |
| GSB (Bivalent) | 3.13e5 | 8.65e-4 | 375 | 3.62e8 | 2.71e-9 | 21.4 |