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RNA promotes stable dimerization of the PatMC/Lsm1–7 complex. (A,B) SEC-MALS of 20.6 µM PatMC/Lsm1–7 with stoichiometric (A), A15 and (B), A10 RNA. Expected and observed molar masses are shown in Table 1. The light scattering (blue) and molar mass (red) are displayed. (C) SV-AUC of 9.3 µM PatMC/Lsm1–7 with stoichiometric A15 RNA at 250 mM NaCl. c(S) is the sedimentation distribution with molecular weights determined from fits.
