FIGURE 4.

The PatMC/Lsm1–7 complex can intrinsically form a dimeric complex. (A) Preparative size exclusion chromatography of the PatMC/Lsm1–7 complex in a 400 mM NaCl buffer. (B) SEC-MALS of the concentrated PatMC/Lsm1–7 complex at 250 mM NaCl. The expected mass of the monomeric PatMC/Lsm1–7 complex is 133 kDa. Expected and observed molar masses are shown in Table 1. The light scattering (blue) and molar mass (red) are displayed. (C) Representative SDS-PAGE gel of peaks (left to right: earlier to later elution volumes). (D) SV-AUC of 9.3 µM PatMC/Lsm1–7 at 250 mM NaCl. c(S) is the sedimentation distribution with molecular weights determined from fits.