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. 2020 Oct;26(10):1380–1388. doi: 10.1261/rna.075812.120

FIGURE 5.

FIGURE 5.

Oligomerization of PatMC promotes liquid–liquid phase separation with Dcp2 and recruitment of additional RNA decay machinery. (A) Schematic of Dcp2 construct used. Purple represents the globular domain that cleaves the m7G cap. Gray bars represent helical leucine motifs in the disordered carboxy-terminal extension. (B) Brightfield and fluorescence microscopy of droplets with 2.5 µM Pat constructs (0.1 µM FITC-labeled) with stoichiometric amounts of Dcp1/Dcp2Ext. (C) Brightfield and fluorescence microscopy of droplets with 2.5 µM PatMC and 2.5 µM rA15 RNA or 13.4 ng/µL poly(A) RNA with or without 2.5 µM Dcp1/Dcp2Ext and 0.1 µM FAM-rA15. (D) Brightfield and fluorescence microscopy of 2.5 µM PatMC (0.1 µM FITC-labeled) and Dcp1/Dcp2Ext with 0.1 µM Alexa555 labeled Lsm1–7. All images taken at 40× magnification. Scale bar, 10 µm.