FIGURE 3.

Complex scenarios are possible for ribosome or polymerase associated proteins. (A) Map from Figure 2 indicating locations of protein candidates in further panels. In quadrant II, 70S ribosomes disintegrated and proteins shifted toward the LMW fractions corresponding to 30S and 50S fractions, increasing the distribution in the gradient. In quadrant III, translation factors occupied subunit fractions, shifting from LMW fractions to ribosomal fractions. In quadrant IV, a number of factors pelleted upon RNase treatment that may require RNA for integrity. An interesting example was the SRP protein, but also some transcription and translation factors were shifted to HMW fractions. (B) The RNAP complex did not shift. RNAP proteins correlated with 6S RNA (RNA sedimentation profiles are represented as blue bars) (Smirnov et al. 2016). The σ S factor shifted to LMW fractions. Interestingly, transcription factors shifted between polymerase-associated fractions; for example, RtcR that was allocated before RNase treatment closely to rtcR RNA (Smirnov et al. 2016). (C) The trans-translation factor SmpB shifted to polymerase associated fractions after RNase treatment, and partly overlapped with its associated tmRNA (Smirnov et al. 2016).