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. 2020 Oct;26(10):1464–1480. doi: 10.1261/rna.075655.120

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© 2020 Hurtig et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Alternative splicing of NUP116/NUP100 orthologs in the Saccharomycotina. (A) Splicing pattern is shown for each of the species analyzed, along with the species phylogeny as in Figure 1A. (B) C. jadinii diverged before the likely origin of NUP116/NUP100 alternative splicing and its ortholog lacks an intron. The C. jadinii ortholog contains a sequence that is homologous to the sequence in Nup116 that binds to Gle2. (C) Intron retention in the NUP116/NUP100 ortholog of L. kluyveri. (D) Intron retention in the NUP116/NUP100 ortholog of T. delbrueckii. (E,F) Duplicated NUP116 and NUP100 genes in S. cerevisiae lack introns and only Nup116 contains the Gle2 binding site.