FIGURE 3.

Lesser accumulation of mRNAs of the RP genes in the absence of Bud27. (A) Analysis of the mRNA accumulation of several RP genes by RT-qPCR in the wild-type and bud27Δ strains. Cells were grown in YPD medium at 30°C and shifted to 37°C for 12 h. The mRNA levels of PYK1 were used as a control of a gene whose expression decreases and those of ACT1 as the control of a gene with no significant alteration. The used oligonucleotides are listed in Supplemental Table S1. (B) Analysis by RT-qPCR of the mRNA accumulation of the immature and mature forms of some intron-containing genes in the wild-type and bud27Δ strains. Cells were grown in YPD medium at 30°C and shifted to 37°C for 12 h. (C) Ratios for the immature versus mature forms of the genes analyzed in B.