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. 2020 Oct;26(10):1389–1399. doi: 10.1261/rna.075135.120

FIGURE 5.

FIGURE 5.

Reactivity plots of enriched U1 snRNAs and nuclear extract U1 snRNA. Black bars (0–0.3) signify background reactivity, orange bars (0.3–0.7) signify intermediate reactivity, and red bars (≥0.7) indicate highly reactive nucleotides. The y-axis represents the normalized SHAPE reactivity and the x-axis indicates the nucleotide position from 5′ to 3′ in the U1 snRNA sequence. Loop nucleotides for stem–loops I and II are indicated in addition to nucleotides that display different SHAPE reactivities between enriched conditions and the NE condition. The reactive nucleotides for stem–loop III are also indicated.