Figure 4. Grafted SC-β Cells Resemble Primary Human β Cells Compared to Stage 6 SC-β Cells.
(A) Unsupervised tSNE projection from 10 islet datasets including stage 6 hiPSC- and hESC-islets, three grafted hESC-islets, two grafted hiPSC-islets, and three primary HIs.
(B) Individual location of different samples within combined tSNE projection.
(C) Volcano plot displaying FC differences of β Cell genes between grafted SC-β (left) and HI β Cells (right). Dashed lines are drawn to define restriction of log FC value of 0.25 and −log of adjusted p value 0.001. Key β Cell genes are labeled and colored red.
(D) Heatmap showing gene expression values for stage 6, grafted SC-β, and primary HI (donor) β Cells of the 100 most differentially expressed genes between stage 6 and grafted SC-β Cells listed in Table S6. Scale: normalized expression.
(E) Pearson correlation matrix and hierarchical clustering to identify most similar populations among all β Cell samples using top 430 variable genes. Pearson correlation values detailed in Table S6. Scale: correlation coefficient.