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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: Cell Rep. 2020 Aug 25;32(8):108067. doi: 10.1016/j.celrep.2020.108067

Figure 6. Analysis of Pseudo-Time and Single-Cell Transcriptomes of Stage 6 hPSC-ECs and Grafted hPSC-EC Cells Following Trans plantation.

Figure 6.

(A) Heatmap of TPH1 gene expression to distinguish SC-EC cell population within tSNE plot from Figure 4A.

(B) Gene expression values for two stage 6 SC-EC cells and five grafted SC-EC cells for the 100 most differentially expressed genes between stage 6 and grafted SC-EC cells listed in Table S6. Scale: normalized expression.

(C) Pearson correlation matrix and hierarchical clustering to identify most similar populations among all EC cell samples using top 430 variable genes. Scale: correlation coefficient.

(D) Expression values for key EC cell identity, function, development, and newly identified genes between stage 6 and grafted SC-EC cells.

(E) Immunostaining of stage 6 and 6-month kidney graft containing SC-islets for EC cell marker TPH1. Scale bar, 50 μm.

(F and G) Distribution (F) of SC-EC cells along pseudo-time trajectory (G) from seven SC-EC samples.

(H) Relative expression of select EC cell genes along SC-EC cell pseudo-time. Scale: relative expression. Each dot represents a different cell.

TPH1, tryptophan hydroxylase 1. See also Figure S6 and Table S4.