KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti C-peptide | DSHB | Cat#GN-ID4; RRID:AB_2255626 |
| Mouse anti NKX6–1 | DSHB | Cat#F55A12; RRID:AB_532379 |
| Rabbit anti Glucagon | Cell Marque | Cat#259A-18; RRID:AB_1158356 |
| Goat anti PDX1 | R&D Systems | Cat#AF2419; RRID:AB_355257 |
| Rabbit anti CHGA | ABCAM | Cat#ab15160; RRID:AB_301704 |
| Rabbit anti MAFA | LifeSpan Bioscience | Cat#LP9872; RRID:AB_2665528 |
| Rabbit anti FAM159B | Invitrogen | Cat#PA5–52855; RRID:AB_2641287 |
| Rabbit anti NAA20 | Invitrogen | Cat#PA5–66574; RRID:AB_2663038 |
| Rabbit anti IAPP | Sigma Aldrich | Cat#PA5–84142; RRID:AB_2791294 |
| Sheep anti ARX | Invitrogen | Cat#PA5–47896; RRID:AB_2605931 |
| Sheep anti TPH1 | EMD Millipore | Cat#AB1541; RRID:AB_90754 |
| Sheep anti Trypsin | R&D | Cat#AF3586; RRID:AB_2846900 |
| Mouse anti KRT19 | Dako | Cat#M0888; RRID:AB_2234418 |
| anti-rat-alexa fluor 488 | Invitrogen | Cat#a21208; RRID:AB_141709 |
| anti-rabbit-alexa fluor 488 | Invitrogen | Cat#a21206; RRID:AB_2535792 |
| anti-rat-alexa fluor 488 | Invitrogen | Cat#a21208; RRID:AB_141709 |
| anti-mouse-alexa fluor 594 | Invitrogen | Cat#a21203; RRID:AB_141633 |
| anti-rabbit-alexa fluor 594 | Invitrogen | Cat#a21207; RRID:AB_141637 |
| anti-goat-alexa fluor 594 | Invitrogen | Cat#a11058; RRID:AB_2534105 |
| anit-sheep-alexa fluor 594 | Invitrogen | Cat#a11016; RRID:AB_10562537 |
| Biological Samples | ||
| Healthy Human Islets | Prodo Labs | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| mTeSR1 | StemCell Technologies | Cat#05850 |
| Y27632 | Abcam | Cat#ab120129 |
| Matrigel | Corning | Cat#356230 |
| TrypLE | Life Technologies | Cat#12-604-039 |
| DNase | QIAGEN | Cat#79254 |
| HEPES | GIBCO | Cat#15630–080 |
| Tris | MilliporeSigma | Cat#T6066 |
| EDTA | Ambion | Cat#327371000 |
| Paraformaldehyde | Electron Microscopy Science | Cat#15714 |
| Donkey Serum | Jackson Immunoresearch | Cat#017-000-121 |
| Triton X-100 | Acros Organics | Cat#327371000 |
| DMEM | MilliporeSigma | Cat#D6429 |
| Fetal Bovine Serum | MilliporeSigma | Cat#F4135 |
| Histo-clear II | Electron Microscopy Sciences | Cat#64111–04 |
| Histogel | Thermo Scientific | Cat#Hg-4000–012 |
| 0.5 M EDTA | Ambion | Cat#AM9261 |
| 200 proof ethanol | Decon | Cat#2716 |
| STZ | R&D | Cat#1621500 |
| Critical Commercial Assays | ||
| Ultrasensitive C-Peptide Elisa | Mercodia | Cat#10-1141-01 |
| Human Amylin Elisa | Miltenyi | Cat#EZHA-52K |
| RNeasy Mini Kit | QIAGEN | Cat#74016 |
| High Capacity cDNA Reverse Transcriptase Kit | Applied Biosystems | Cat#4368814 |
| PowerUp SYBR Green Master Mix | Applied Biosystems | Cat#A25741 |
| Chromium Single Cell 3’ Library &Gel Bead Kit | 10X Genomics | Cat#PN-120267 |
| Deposited Data | ||
| raw and processed single cell RNA seq data - Stage 6 hESC-islet, 3 Grafted hESC-islet, 2 Grafted hiPSC-islet | This Paper | GEO: GSE151117 |
| raw and processed single cell RNA seq data - Stage 6 hiPSC-islet | Maxwell et al., 2020 | GEO: GSE139535 |
| raw and processed single cell RNA seq data - Human Islets | Xin etal., 2018 | GEO: GSE114297 |
| Experimental Models: Cell Lines | ||
| Human: HUES8 hESC | HSCI | hES Cell Line: HUES-8 |
| Human: WS4corr | Maxwell et al., 2020 | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wj/SzJ (NSG) | The Jackson Laboratories | JAX: 005557; RRID:IMSR_JAX:005557 |
| Oligonucleotides | ||
| Primers for this study, see Table S1 | This paper | N/A |
| Software and Algorithms | ||
| Fiji ImageJ | ImageJ public freeware | https://imagej.net/Fiji/Downloads; RRID:SCR_003070 |
| GraphPad Prism 8.2.1 | GraphPad |
https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| FLOWJO | FLOWJO | https://www.flowjo.com/solutions/flowjo/downloads; RRID:SCR_008520 |
| GSEA 4.0.2 | GSEA | https://www.gsea-msigdb.org/gsea/index.jsp; RRID:SCR_003199 |
| RStudio | RStudio | https://rstudio.com/products/rstudio/download/; RRID:SCR_000432 |
| R | R | https://cran.r-project.org/src/base/R-4/ |
| Seurat v2.3.4 | Butler et al., 2018 | https://satijalab.org/seurat/install.html; RRID:SCR_007322 |
| Monocle v2.12.0 | Qiu et al., 2017 | http://cole-trapnell-lab.github.io/monocle-release/docs/#installing-monocle |
| Other | ||
| Vi-Cell XR | Beckman Coulter | Cat#Vi-Cell XR |
| Tanswells | Corning | Cat#431752 |
| #1.5 glass bottom 96 well plate | Cellvis | Cat#963–1.5H-N |
| 8-channel peristaltic pump | ISMATEC | Cat#ISM931C |
| Inlet/outlet two-stop tubing | ISMATEC | Cat#070602–04i-ND |
| Dispensing nozzle | BioRep | Cat#Peri-Nozzle |
| Connection tubbing | BioRep | Cat#Peri-TUB-040 |
| Bio-Gel P-4 | Bio-Rad | Cat#150–4124 |
| Cell Chamber | BioRep | Cat#Peri-Chamber |
| Pressure cooker | Electron Microscopy Sciences | Model#Retriever 2100 |
| DAPI Fluoromount-G | SouthernBiotech | Cat#0100–20 |
| Phosphate-Buffered Saline (PBS) | Corning | Cat#21–040-CV |
| micro coverslip | VWR | Cat#48393–081 |