Fig. 2.

Ae inhibits PEDV replication in vitro. Vero or IPEC-J2 cells were treated with various concentrations of Ae (4-16 mg/mL) or the control normal DMEM for 1 h, followed by infection with PEDV at an MOI of 0.2 or 0.4. After 1 h, the cells were re-treated with Ae of DMEM as control. (A) At 24 h postinoculation, an indirect immunofluorescence assay was performed. CPE and PEDV antigen were indicated by arrows. Vero or IPEC-J2 cells were treated as above described, at indicated time points (12 h, 24 h and 48 h), cell lysates were prepared and examined with Western Blot using anti-PEDV N polyclonal antibody (B) or the viral titers in the cell lysates were determined by TCID₅₀ analysis (C). Results are representative of three independent experiments. Data are represented as mean ± SD, n = 3.