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. Author manuscript; available in PMC: 2021 Jun 24.
Published in final edited form as: Chem Rev. 2020 Mar 4;120(12):5107–5157. doi: 10.1021/acs.chemrev.9b00704

Table 8.

Hybrid Enzymes and the Reported Reactivitya

Products Observed
Protein
(cell background)		 Hybridb Methodc NH3 C2H4 C2H6 H2 ref
Kp NifDK
(ΔnifB) 		V-MoFe Apo-NifDK cell extract or isolated protein incubated with V-cluster ND + + + 157
Av NifDK
(ΔnifB)		 V-MoFe Apo-NifDK cell extract incubated with V-cluster NR + + NR 157
Av VnfDGK
(ΔnifHDKB)		 M-VFe Apo-VnfDGK cell extract incubated with M-cluster + + + + 163
Av NifDK
(ΔnifB)		 V-MoFe Isolated apo-NifDK incubated with V-cluster + + + + 168,222
Av VnfDGK
(ΔnifHDKB)		 M-VFe Isolated apo-VnfDGK incubated with M-cluster + + NR + 34
Av VnfDGK
(ΔnifHDK)		 M-VFe Cells grown with excess Na2MoO4 (−Na3VO4) + + + + 23
Av NifDK
(W-resistant)		 W/Mo-MoFe Cells grown with Na2WO4 (−Na2MoO4) + + NR + 224
Rc NifDK
(FeFe-suppressed)		 W-MoFe Cells grown with Na2WO4 (−Na2MoO4) ND + NR + 223
a

ND = not detected. NR = not reported. Plus (+) sign indicates that the activity is observed/reported.

b

the nomenclature of the hybrid protein is listed as ‘cluster-protein’ where cluster can be the M, V or W-clusters and the protein is NifDK (also called MoFe protein) or VnfDGK (also called VFe protein). Thus, V-MoFe refers to the hybrid protein that has the Mo nitrogenase polypeptide (NifDK) with the V nitrogenase cofactor (V-cluster).

c

the description for the method used to obtain the hybrid protein