. Author manuscript; available in PMC: 2021 Jun 24.

Published in final edited form as: Chem Rev. 2020 Mar 4;120(12):5107–5157. doi: 10.1021/acs.chemrev.9b00704

Table 9.

Substrate Reduction Activity Facilitated by NifH and VnfH^a

			Product
Protein	Redox Agent	Nucleotide	CO^b	CO₂^c
Av NifH	DT	-	0.10	-
	DT	+	0.15	-
	IDS	-	0.0056	200
	Eu^IIDTPA	-	3.2/7.7^d	-
	Eu^IIDTPA	+	7.6^d	-
Av VnfH	DT	-	0.13	-
	DT	+	0.17	-
	IDS	-	0.0083	190
	Eu^IIDTPA	-	3.2/7.8^d	-
	Eu^IIDTPA	+	7.6^d	-

The data is reported from ref 87, all proteins are from A. vinelandii. Units reported as 10⁻³ nmol product × min⁻¹ × (nmol protein)⁻¹. All reactions reported at 30 °C. DT = sodium dithionite. IDS = indigo disulfonate.

assay reported in 100% CO₂ atmosphere.

assay reported in 99.9% CO atmosphere.

CO production after multiple additions of Eu^II reductant; values reported with units of nmol CO × (nmol protein)⁻¹.