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. Author manuscript; available in PMC: 2021 Aug 9.
Published in final edited form as: Alcohol Clin Exp Res. 2020 Aug 9;44(8):1561–1570. doi: 10.1111/acer.14399

Figure 2. GRIK1 gene expression in A/A and C/C iPSC-derived neural cultures.

Figure 2.

(A) Schematic representing the two major GRIK1 isoforms, which differ by the exclusion of exons 9 and 17 in isoform 2 (accession ID U16125), and exclusion of exon 18 in isoform 1 (accession ID NM-000830) as well as the antisense-2 RNA (Ref Seq accession ID NR_033368.1; which is also annotated as BACH1-207 in the Ensemble transcript database – ENST00000462262.1). Approximate location of rs2832407 is indicated by the yellow dot, which resides in the intronic region between exons 9-10 in isoform 1 and 8-10 in isoform 2. (B-F) iPSC-derived neural cultures were examined 12 weeks post plating. No significant effect of rs2832407 genotype was observed on the expression of GRIK1 isoform 1 (B), isoform 2 (C, D), or total GRIK1 (E). A significant effect of genotype was observed on the expression of GRIK1 antisense-2 (F). Each dot represents the average RNA expression collected from a donor subject.