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. Author manuscript; available in PMC: 2021 Aug 9.
Published in final edited form as: Alcohol Clin Exp Res. 2020 Aug 9;44(8):1561–1570. doi: 10.1111/acer.14399

Figure 3. Topiramate exposure attenuates the frequency of spontaneous excitatory synaptic activity.

Figure 3.

(A) Example trace representing the spontaneous excitatory postsynaptic currents (EPSCs) that were quantified, which were validated as excitatory by observing their attenuation in the presence of the AMPA/kainate receptor antagonist DNQX (10 μM). (B) Representative traces of EPSCs recorded from an A/A neuron and C/C neuron in the absence and presence of 5 μM topiramate for 20 min. (C) 12+ week old neurons generated from 3 A/A and 4 C/C donors were exposed to 5 μM topiramate for 30 min, and EPSCs were recorded and frequency plotted in 10 min bins. There was a significant interactive effect of topiramate exposure time and GRIK1 genotype on the frequency of EPSCs. Neurons derived from A/A donors showed a larger reduction in the frequency of EPSCs than neurons derived from C/C donors. Each dot represents an individual neuron. Thick lines represent the mean frequency per genotype. Thin lines depict ± SEM.