| Antibodies |
|
|
| HA (rabbit) |
Abcam |
Cat# ab9110 RRID:AB_307019Rabbit |
| HA (rat) |
Roche |
Cat# 11867423001, RRID:AB_390918 |
| NLGN4 |
Abcam |
Cat#: ab181251 |
| Myc |
Cell Signaling Technology |
Cat# 2276, RRID:AB_331783 |
| Actin |
Abm |
Cat# G043 |
| GFP |
Thermo Fisher |
Cat# A10262, RRID:AB_2534023 |
| pan-NLGN |
Synaptic System |
Cat# 129 011, RRID:AB_887745 |
| BiP |
BD Biosciences |
Cat# 610978, RRID:AB_398291 |
| Tau |
Synaptic System |
Cat# 314 011, RRID:AB_10805762 |
| Transferrin Receptor |
Thermo Fisher |
Cat# 13–6800, RRID:AB_2533029 |
| VGLUT1 |
Millipore |
Cat# AB5905, RRID:AB_2301751 |
| Mouse IgG HRP-linked |
GE Healthcare |
Cat#: NA931 RRID:AB_772210 |
| Rabbit IgG HRP-linked |
GE Healthcare |
Cat#: NA934 RRID:AB_772206 |
| Anti-chicken Alexa Fluor 488 |
Thermo Fisher |
Cat# A-11039, RRID:AB_2534096 |
| Anti-rabbit Alexa Fluor 488 |
Thermo Fisher |
Cat#:A11034 RRID:AB_2576217 |
| Anti-mouse Alexa Fluor 405 |
Thermo Fisher |
Cat# A-31553, RRID:AB_221604 |
| Anti-rabbit Alexa Fluor 555 |
Thermo Fisher |
Cat#: A21428 RRID:AB_141784 |
| Anti-rat Alexa Fluor 647 |
Thermo Fisher |
Cat# A-21247, RRID:AB_141778 |
| Anti-guinea pig Alexa Fluor 647 |
Thermo Fisher |
Cat# A-21450, RRID:AB_2735091 |
| NLGN4Y |
This study |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Lipofectamine 2000 |
Thermo Fisher |
Cat# 11668019 |
| Protease inhibitor mixture |
Roche |
Cat#: 11697498001 |
| Phosphatase inhibitor mixture II |
Sigma-Aldrich |
Cat#: P5726 |
| Phosphatase inhibitor mixture III |
Sigma-Aldrich |
Cat#: P0044 |
| Protein A-Sepharose |
Sigma-Aldrich |
Cat#: P3391 |
| Cell Lines |
|
|
| Human embryonic kydney (HEK) 293T |
ATCC |
Cat#: CRL11268 RRID:CVCL_1926 |
| COS 7 |
ATCC |
Cat# CRL-1651, RRID:CVCL_0224 |
| Organisms/Strains |
|
|
| Sprague Dawley outbred rat |
Envigo |
Cat#: 002 |
| Oligonucleotides |
|
|
| pCAG-NLGN4X-Myc FW primer GAACAAAAACTCATCTCAGAAGAGGATCTGCCAGTTGTCAACACAAATT |
This study |
N/A |
| pCAG-NLGN4X-Myc RW primer CAGATCCTCTTCTGAGATGAGTTTTTGTTCATACTGTGCTTGGCTGTCAATGA |
This study |
N/A |
| pCAG-NLGN4X-HA R56Q FW primer CAAATTATGGCAAAATCCAGGGCCTAAGAAC |
This study |
N/A |
| pCAG-NLGN4X-HA R56Q RW primer GGTGTTCTTAGGCCCTGGATTTTGCC |
This study |
N/A |
| pCAG-NLGN4X-HA N64S FW primer TAAGAACACCGTTACCCAGTGAGATCTTGGGTCCA |
This study |
N/A |
| pCAG-NLGN4X-HA N64S RW primer TGGACCCAAGATCTCACTGGGTAACGGTGTTCTTA |
This study |
N/A |
| pCAG-NLGN4X-HA P93S FW primer GCCCCCAGAAAGCCCGTCC |
This study |
N/A |
| pCAG-NLGN4X-HA P93S RW primer GAGGACGGGCTTTCTGGGGG |
This study |
N/A |
| pCAG-NLGN4X-HA E85D FW primer CACCCCCCACTGGAGATAGGCGGTTTC |
This study |
N/A |
| pCAG-NLGN4X-HA E85D RW primer GAAACCGCCTATCTCCAGTGGGGGGTG |
This study |
N/A |
| pCAG-NLGN4X-HA G84R FW primer CTCACCCCCCACTAGAGAGAGGCG |
This study |
N/A |
| pCAG-NLGN4X-HA G84R RW primer CGCCTCTCTCTAGTGGGGGGTGAG |
This study |
N/A |
| pCAG-NLGN4X-HA R87W FW primer CACTGGAGAGAGGTGGTTTCAGCCCC |
This study |
N/A |
| pCAG-NLGN4X-HA R87W RW primer GGGGCTGAAACCACCTCTCTCCAGTG |
This study |
N/A |
| pCAG-NLGN4X-HA P94L FW primer CCCAGAACCCCTGTCCTCCTGGAC |
This study |
N/A |
| pCAG-NLGN4X-HA P94L RW primer GTCCAGGAGGACAGGGGTTCTGGG |
This study |
N/A |
| pCAG-NLGN4X-HA G99S FW primer CCCGTCCTCCTGGACTAGCATCCGAAATAC |
This study |
N/A |
| pCAG-NLGN4X-HA G99S RW primer GTATTTCGGATGCTAGTCCAGGAGGACGGG |
This study |
N/A |
| pCAG-NLGN4X-HA/Myc R101Q FW primer CTCCTGGACTGGCATCCAAAATACTACTCAGTTTG |
This study |
N/A |
| pCAG-NLGN4X-HA/Myc R101Q RW primer CAAACTGAGTAGTATTTTGGATGCCAGTCCAGGAG |
This study |
N/A |
| pCAG-NLGN4X-HA/Myc V109L FW primer CTACTCAGTTTGCTGCTTTGTGCCCCCAG |
This study |
N/A |
| pCAG-NLGN4X-HA/Myc V109L RW primer CTGGGGGCACAAAGCAGCAAACTGAGTAG |
This study |
N/A |
| pCAG-NLGN4Y-HA FW primer TATCCATACGACGTTCCGGACTACGCTCCAGTTGTCAACACAAATTATGC |
This study |
N/A |
| pCAG-NLGN4Y-HA RW primer AGCGTAGTCCGGAACGTCGTATGGATAATACTGTGCTTGGCTGTCAATGAG |
This study |
N/A |
| pCAG-NLGN4Y-Myc FW primer GAACAAAAACTCATCTCAGAAGAGGATCTGCCAGTTGTCAACACAAATT |
This study |
N/A |
| pCAG-NLGN4Y-Myc RW primer CAGATCCTCTTCTGAGATGAGTTTTTGTTCATACTGTGCTTGGCTGTCAATGA |
This study |
N/A |
| pCAG-NLGN4Y-HA S93P FW primer GTTTCAGCCACCAGAACCCCCATCCTC |
This study |
N/A |
| pCAG-NLGN4Y-HA S93P RW primer GAGGATGGGGGTTCTGGTGGCTGAAAC |
This study |
N/A |
| pCAG-NLGN4X/Y or NLGN4Y/X-HA FW primer GCCTTTGCGGCGCTGTACTACAAAA |
This study |
N/A |
| pCAG-NLGN4X/Y or NLGN4Y/X-HA RW primer TTTTGTAGTACAGCGCCGCAAAGGC |
This study |
N/A |
| pCAG-NLGN4X-SP-Y or NLGN4Y-SP-X-HA FW primer CCTCATTGACAGCCAAGCACAG |
This study |
N/A |
| pCAG-NLGN4X-SP-Y or NLGN4Y-SP-X-HA RW primer CTGTGCTTGGCTGTCAATGAGG |
This study |
N/A |
| pCAG-NLGN4X-100-Y or NLGN4Y-100-X-HA FW primer CATGGGGGATCTTACATGGAGG |
This study |
N/A |
| pCAG-NLGN4X-100-Y or NLGN4Y-100-X-HA RW primer CCTCCATGTAAGATCCCCCATG |
This study |
N/A |
| pCAG-NLGN4X-180-Y or NLGN4Y-180-X-HA FW primer TCCTCCTGGACTGGCATCC |
This study |
N/A |
| pCAG-NLGN4X-180-Y or NLGN4Y-180-X-HA RW primer GGATGCCAGTCCAGGAGGA |
This study |
N/A |
| NLGN4X/Y RT-PCR primer FW CGACGTTCCGGACTACGCTCCAGTTG |
This study |
N/A |
| NLGN4X/Y RT-PCR primer RW GTAAAGGCAGTCTTCATTTTGATCTTGAAC |
This study |
N/A |
| GAPDH RT-PCR primer FW CCAGCCGAGCCACATCGCTC |
This study |
N/A |
| GAPDH RT-PCR primer RW ATGAGCCCCAGCCTTCTCCAT |
This study |
N/A |
| Recombinant DNA |
|
|
| pCAG-NLGN4X-HA |
Bemben et al., 2014 |
N/A |
| NLGN4Y |
Origene |
Cat#: SC31009 |
| pCAG-NLGN4Y-HA |
Bemben et al., 2015 |
N/A |
| pCAG-NLGN4Y-Myc |
This study |
N/A |
| pCAG-NLGN1-HA |
Bemben et al., 2019 |
N/A |
| pCAG-NLGN2-HA |
Bemben et al., 2019 |
N/A |
| pCAG-NLGN3-HA |
Bemben et al., 2019 |
N/A |
| pCAG-NLGN4X/4Y-HA |
This study |
N/A |
| pCAG-NLGN4Y/4X-HA |
This study |
N/A |
| pCAG-NLGN4X-180-Y-HA |
This study |
N/A |
| pCAG-NLGN4Y-180-X-HA |
This study |
N/A |
| pCAG-NLGN4X-100-Y-HA |
This study |
N/A |
| pCAG-NLGN4Y-100-X HA |
This study |
N/A |
| pCAG-NLGN4X-SP-Y-HA |
This study |
N/A |
| pCAG-NLGN4Y-SP-X-HA |
This study |
N/A |
| ER-Crimson |
Addgene |
Cat#: 104010 |
| pCAG-NLGN4X R56Q-HA |
This study |
N/A |
| pCAG-NLGN4X N64S-HA |
This study |
N/A |
| pCAG-NLGN4X P93S-HA |
This study |
N/A |
| pCAG-NLGN4X G84R-HA |
This study |
N/A |
| pCAG-NLGN4X R87W-HA |
This study |
N/A |
| pCAG-NLGN4X P94L-HA |
This study |
N/A |
| pCAG-NLGn4X G99S-HA |
This study |
N/A |
| pCAG-NLGN4X R101Q-HA |
This study |
N/A |
| pCAG-NLGN4X V109L-HA |
This study |
N/A |
| pCAG-NLGN4Y S93P-HA |
This study |
N/A |
| pCAG-NLGN4X-Myc |
This study |
N/A |
| pCAG-NLmiRs-GFP |
Bemben et al., 2014 |
N/A |
| pCAG-eGFP |
Bemben et al., 2014 |
N/A |
| CD4 |
Li et al., 2017 |
N/A |
| Software and Algorithms |
|
|
| Adobe Photoshop CC 2018 |
Adobe |
RRID:SCR_014199 |
| Adobe Illustrator CC 2018 |
Adobe |
RRID:SCR_010279 |
| Fiji |
NIH |
RRID:SCR_002285 |
| GraphPad Prism 8 |
Graphpad |
RRID:SCR_002798 |
| Proteome Discover 2.2 |
Thermo Fisher |
Cat# 30795 RRID:SCR_014477 |
| Metamorph |
Molecular Devices |
RRID: SCR_002368 |
