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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: Neuroscience. 2019 Jun 14;413:230–238. doi: 10.1016/j.neuroscience.2019.05.062

Figure 3. Reduce expression of Vimentin, Pax6, and proliferation suggest a loss of radial glial stem cell in Jag1 mutants.

Figure 3.

(A-B) RNA In situ hybridization using a Vimentin probe and (C-D) Immunohistochemistry using anti-Pax6 antibody on sagittal sections show thinner expression in Jag1 mutant. Quantification of Vimentin (I) and Pax6+ (J) labeling shows decrease expression in mutants compared to control. Antibodies against p-HH3 (E-F, K) and Ki67 (G-H, L) show decrease expression in the VZ. Scale bar = 200μm. All graphs show means ± SEM. *, p<0.05; **, p<0.01; ***, p<0.001. Cx, cortex; dLGE, dorsal lateral ganglionic eminence.