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. 2020 Sep 15;15(9):e0238647. doi: 10.1371/journal.pone.0238647

Fig 6. Data collection workflow.

Fig 6

(1) HDFa cells are seeded onto 12-well plate inserts. (2) Cultured overnight until >90% confluent. (3) Treated with cell trauma inducing agent. (4) Incubated for 1–4 hours dependent upon cell trauma agent. (5) Cell staining to confirm apoptosis/necrosis levels. (6) Reduction of growth medium to 0.5 mL for imaging preparation. (7) Transfer to imaging box for NCI data collection. (8) Image collection using NCI SWIR/MWIR detector. (9) Images analysed to produce cell spectral data. (10) Pre- and post-process spectral analysis.